abstract：:Liver disease is an increasing global health burden. The final sequalae of cirrhosis, liver failure and hepatocellular carcinoma are often the result of inflammation driven by intrahepatic lymphocytes. Accurate assessment of organ-specific diseases ideally employs tissue sampling though this is rarely performed. Here ...

journal_title：Journal of immunological methods

authors： Pembroke T,Gallimore A,Godkin A

更新日期：2015-09-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) have emerged as a promising tool for cancer therapy. Differing approaches utilize mAbs to either deliver a drug to the tumor cells or to modulate the host's immune system to mediate tumor kill. The rate by which a therapeutic antibody is being internalized by tumor cells is a decisive feat...

journal_title：Journal of immunological methods

authors： Hazin J,Moldenhauer G,Altevogt P,Brady NR

更新日期：2015-08-01 00:00:00

abstract：:Visceral leishmaniasis, which is also known as Kala-Azar, is one of the most severely neglected tropical diseases recognized by the World Health Organization (WHO). The threat of this debilitating disease continues due to unavailability of promising drug therapy or human vaccine. An extensive research is undergoing to...

journal_title：Journal of immunological methods

authors： Jain K,Jain NK

更新日期：2015-07-01 00:00:00

abstract：:Recently the analytical power of the latest high throughput next generation DNA sequencing platforms has been used to analyse phage that have been selected from the panning of large combinatorial libraries displaying either peptide or antibody ligands. This process, commonly referred to as next generation phage displa...

journal_title：Journal of immunological methods

authors： Spiliotopoulos A,Owen JP,Maddison BC,Dreveny I,Rees HC,Gough KC

更新日期：2015-05-01 00:00:00

abstract：:Anti-drug neutralizing antibodies (NAbs) formed due to unwanted immunogenicity of a therapeutic protein point towards a mature immune response. NAb detection is important in interpreting the therapeutic's efficacy and safety in vivo. In vitro cell-based NAb assays provide a physiological system for NAb detection, howe...

journal_title：Journal of immunological methods

authors： Hu J,Wala I,Han H,Nagatani J,Barger T,Civoli F,Kaliyaperumal A,Zhuang Y,Gupta S

更新日期：2015-04-01 00:00:00

abstract：:HIV-1 negative regulatory factor (Nef) can inhibit CTL recognition by downregulation of HLA-A and HLA-B on the cell surface. In contrast, HLA-C is not affected by Nef and a growing number of studies demonstrate an important role of HLA-C for the control of HIV-1. So far, only a limited number of HLA-C restricted CTL e...

journal_title：Journal of immunological methods

authors： Stoll A,Bergmann S,Mummert C,Mueller-Schmucker SM,Spriewald BM,Harrer EG,Harrer T

更新日期：2015-03-01 00:00:00

abstract：:The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historica...

journal_title：Journal of immunological methods

authors： Gene RW,Kumaran J,Aroche C,van Faassen H,Hall JC,MacKenzie CR,Arbabi-Ghahroudi M

更新日期：2015-01-01 00:00:00

abstract：INTRODUCTION:Infection and sepsis are major health problems. Therefore, the need for improved diagnostic indicators, as well as for better therapeutic monitors in the treatment of infection, remains, since the current diagnostic tools have low specificity and passed through minimal changes in the last two decades. OBJ...

journal_title：Journal of immunological methods

authors： Farias MG,de Lucena NP,Dal Bó S,de Castro SM

更新日期：2014-12-01 00:00:00

abstract：:The identification and characterization of human mast cell (MC) functions are hindered by the shortage of MC populations suitable for investigation. Here, we present a novel technique for generating large numbers of well differentiated and functional human MCs from peripheral stem cells (=peripheral stem cell-derived ...

journal_title：Journal of immunological methods

authors： Schmetzer O,Valentin P,Smorodchenko A,Domenis R,Gri G,Siebenhaar F,Metz M,Maurer M

更新日期：2014-11-01 00:00:00

abstract：:The concentrations of major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO) have been associated with eosinophilic disease severity. Whereas a variety of techniques have been used to measure individual eosinophil granule protein concentration,...

journal_title：Journal of immunological methods

authors： Makiya MA,Herrick JA,Khoury P,Prussin CP,Nutman TB,Klion AD

更新日期：2014-09-01 00:00:00

abstract：:Malaria imposes a horrific public health burden - hundreds of millions of infections and millions of deaths - on large parts of the world. While this unacceptable health burden and its economic and social impact have made it a focal point of the international development agenda, it became consensual that malaria contr...

journal_title：Journal of immunological methods

authors： Zuzarte-Luis V,Mota MM,Vigário AM

更新日期：2014-08-01 00:00:00

abstract：:Dengue virus (DENV) has substantial global impact, with an estimated 390million people infected each year. In spite of this, there is currently no approved DENV-specific vaccine or antiviral. One reason for this is the difficulty involved with development of an adequate animal model. While non-human primates support v...

journal_title：Journal of immunological methods

authors： Plummer EM,Shresta S

更新日期：2014-08-01 00:00:00

abstract：:Fungi represent emerging infectious threats to human populations worldwide. Mice and other laboratory animals have proved invaluable in modeling clinical syndromes associated with superficial and life-threatening invasive mycoses. This review outlines salient features of common vertebrate animal model systems to study...

journal_title：Journal of immunological methods

authors： Hohl TM

更新日期：2014-08-01 00:00:00

abstract：:External proficiency testing programs designed to evaluate the performance of end-point laboratories involved in vaccine and therapeutic clinical trials form an important part of clinical trial quality assurance. Good clinical laboratory practice (GCLP) guidelines recommend both assay validation and proficiency testin...

journal_title：Journal of immunological methods

authors： Garcia A,Keinonen S,Sanchez AM,Ferrari G,Denny TN,Moody MA

更新日期：2014-07-01 00:00:00

abstract：:Kidney ischemia-reperfusion injury (IRI) occurs as a result of complex interactions of kidney parenchymal cells and immune cells that are initiated by hypoxic damage of parenchymal cells. In particular, tubular epithelial cells (TECs) not only are susceptible to ischemia but also have an auto-loop system to amplify re...

journal_title：Journal of immunological methods

authors： Kim HJ,Park SJ,Koo S,Cha HJ,Lee JS,Kwon B,Cho HR

更新日期：2014-05-01 00:00:00

abstract：:Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action implicated in the clinical efficacy of several therapeutic antibodies. In vitro ADCC assays employing effector cells capable of inducing lysis of target cells bound by antibodies are routinely performed to support the research and...

journal_title：Journal of immunological methods

authors： Chung S,Lin YL,Reed C,Ng C,Cheng ZJ,Malavasi F,Yang J,Quarmby V,Song A

更新日期：2014-05-01 00:00:00

abstract：INTRODUCTION:Infection with the trematode helminth Schistosoma mansoni affects more than 200 million people worldwide. Infected patients are thought to show a decreased incidence of asthma and autoimmune diseases, which is, among others, considered a result of an increased production of the immunoregulatory cytokine IL...

journal_title：Journal of immunological methods

authors： Scheer S,Gross S,Mouahid G,Moné H,Lamers MC

更新日期：2014-04-01 00:00:00

abstract：:The in vitro assessment of T-cell-mediated cytotoxicity plays an important and increasingly relevant role both in preclinical target evaluation and during immunomonitoring to accompany clinical trials employing targeted immunotherapies. For a long time, the gold standard for this purpose has been the chromium release ...

journal_title：Journal of immunological methods

authors： Peper JK,Schuster H,Löffler MW,Schmid-Horch B,Rammensee HG,Stevanović S

更新日期：2014-03-01 00:00:00

abstract：:Intracellular probes used for oxidative burst evaluation could be a substrate of the multidrug resistance proteins (MDR), which may cause misinterpretation of experimental data. We aimed to study the effect of the culture condition and of the MDR-interfering antioxidant quercetin on free radical measurement in serum d...

journal_title：Journal of immunological methods

authors： Peluso I,Romanelli L,Spagnuolo S,Manafikhi H,Palmery M

更新日期：2014-03-01 00:00:00

abstract：:Vaccination against drugs of abuse shows efficacy in animal models, yet few subjects achieve effective serum antibody titers in clinical studies. A barrier to translation is the lack of pre-vaccination screening assays that predict the most effective conjugate vaccines or subjects amenable to vaccination. To address t...

journal_title：Journal of immunological methods

authors： Taylor JJ,Laudenbach M,Tucker AM,Jenkins MK,Pravetoni M

更新日期：2014-03-01 00:00:00

abstract：:Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are pathogens commonly associated with infectious diseases in childhood. This study aimed to develop a fluorescent multiplexed bead-based immunoassay (FMIA) using recombinant proteins for the quantitation of serum IgG antibodies against these ...

journal_title：Journal of immunological methods

authors： Andrade DC,Borges IC,Laitinen H,Ekström N,Adrian PV,Meinke A,Barral A,Nascimento-Carvalho CM,Käyhty H

更新日期：2014-03-01 00:00:00

abstract：:To compare the responses of individual neutrophils to chemoattractants, migration pathway data were obtained using TAXIScan, an optically accessible/horizontal apparatus in which a concentration gradient is established reproducibly for a given stimulus. The observed linear-mode trajectory pattern of neutrophils toward...

journal_title：Journal of immunological methods

authors： Yamauchi A,Degawa-Yamauchi M,Kuribayashi F,Kanegasaki S,Tsuchiya T

更新日期：2014-02-01 00:00:00

abstract：:Establishment of humoral immunity against pathogens is dependent on events that occur in the germinal center and the subsequent induction of high-affinity neutralizing antibodies. Quantitative assays that allow monitoring of affinity maturation and duration of antibody responses can provide useful information regardin...

journal_title：Journal of immunological methods

authors： Lynch HE,Stewart SM,Kepler TB,Sempowski GD,Alam SM

更新日期：2014-02-01 00:00:00

abstract：:Antibodies to a wide range of self-antigens, including those directed against nucleic acids or nucleic acid-binding proteins are the essential biomarkers for diseases such as systemic lupus erythematosus (SLE). Highly complex libraries of nonamers consisting of N-substituted glycines (peptoids) were screened for compo...

journal_title：Journal of immunological methods

authors： Quan J,Lakhanpal A,Reddy MM,Zaman S,Li QZ,German DC,Olsen NJ,Kodadek T,Karp DR

更新日期：2014-01-15 00:00:00

abstract：:BlockLogo is a web-server application for the visualization of protein and nucleotide fragments, continuous protein sequence motifs, and discontinuous sequence motifs using calculation of block entropy from multiple sequence alignments. The user input consists of a multiple sequence alignment, selection of motif posit...

journal_title：Journal of immunological methods

authors： Olsen LR,Kudahl UJ,Simon C,Sun J,Schönbach C,Reinherz EL,Zhang GL,Brusic V

更新日期：2013-12-31 00:00:00

abstract：:The concept of TH17 stemness is attracting increasing attention in the field of tumor immunology. The expression of stem cell-like properties and the promotion of long-term immunity by TH17 cells are also of outmost relevance for autoimmunity. Studying two mouse models of multiple sclerosis (MS), we show that CNS anti...

journal_title：Journal of immunological methods

authors： Kuerten S,Wunsch M,Lehmann PV

更新日期：2013-12-15 00:00:00

abstract：:The single-chain Fv fragment (scFv) is the most frequently used form of recombinant antibody. It is possible to establish clones specific to a certain target by displaying the scFv library on phages followed by biopanning against the target. For the construction of superior scFv libraries, the light-chain variable reg...

journal_title：Journal of immunological methods

authors： Kato M,Hanyu Y

更新日期：2013-10-31 00:00:00

abstract：:Antigen-specific activation of human B cells represents a key step for the production of monoclonal antibodies. Several approaches have been developed over the last thirty years in order to improve the process of lymphocyte activation in vitro. In the present study, we investigated whether the transcriptional transact...

journal_title：Journal of immunological methods

authors： Ait Mebarek M,Wijkhuisen A,Adel-Patient K,Lamourette P,Léonetti M,Volland H

更新日期：2013-10-31 00:00:00

abstract：:Helicobacter pylori infection is the most common human infection where approximately 50% of the world populations are infected. The diagnosis of such infection is mainly done by endoscopy where gastric biopsies are examined for the presence of H. pylori. Such invasive approach is costly, time consuming and generally r...

journal_title：Journal of immunological methods

authors： Karakus C,Salih BA

更新日期：2013-10-31 00:00:00

abstract：:There are a number of mouse models of allergic airway inflammation used to delineate various aspects of asthma. One of the hurdles with using mice is their natural resistance to developing a Th2 allergic response. This is often overcome by double priming with the allergen and an adjuvant. Here we report on an efficien...

journal_title：Journal of immunological methods

authors： Shaw OM,Harper JL

更新日期：2013-09-30 00:00:00

abstract：:Interferon gamma (IFNγ) is a cytokine involved in many anti-viral and immunoregulatory processes. One of the major mechanisms through which IFNγ exerts these effects is by inducing expression of indoleamine 2,3 dioxygenase-1 (IDO1), an enzyme that catalyses the first, rate-limiting step of the kynurenine pathway. In t...

journal_title：Journal of immunological methods

authors： Jusof FF,Khaw LT,Ball HJ,Hunt NH

更新日期：2013-08-30 00:00:00

abstract：:The characterization of mucins is critically important for gaining insights into the molecular pathology of diseases, including cancers, as well as for the discovery of biomarkers for disease diagnosis. However, no practical method has yet been reported for identifying mucin proteins. Here, we report a technique for i...

journal_title：Journal of immunological methods

authors： Matsuno YK,Dong W,Yokoyama S,Yonezawa S,Narimatsu H,Kameyama A

更新日期：2013-08-30 00:00:00

abstract：:Longitudinal clinical or experimental immunological studies warrant the use of cryopreserved samples for flow cytometric phenotyping. Most notably CD62L+ and CD25+Foxp3+ counts were shown to be reduced by past studies. Here we are the first to compare the effects of cryopreservation on cell type calculations performed...

journal_title：Journal of immunological methods

authors： Ogunjimi B,Hens N,Malfait R,Van Tendeloo V,Smits E

更新日期：2013-06-28 00:00:00

abstract：:Paraneoplastic neurological syndromes (PNS) are devastating neurological disorders secondary to cancer, associated with onconeural autoantibodies. Such antibodies are directed against neuronal antigens aberrantly expressed by the tumor. The detection of onconeural antibodies in a patient is extremely important in diag...

journal_title：Journal of immunological methods

authors： Maat P,Brouwer E,Hulsenboom E,VanDuijn M,Schreurs MW,Hooijkaas H,Smitt PA

更新日期：2013-05-31 00:00:00

abstract：OBJECTIVES:To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro. METHODS:A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro p...

journal_title：Journal of immunological methods

authors： Dellavance A,Alvarenga RR,Rodrigues SH,Barbosa SH,Camilo AC,Shiguedomi HS,Rodrigues SS,Silva CG,Andrade LE

更新日期：2013-04-30 00:00:00

abstract：:Phagocytosis plays a pivotal and essential role in host immune defense, both as a focal constituent of the innate immune response and a bridging element linking innate and adaptive immunity. Phagocytosis has been demonstrated to be critical in development, tissue remodeling, wound healing and resolution of inflammatio...

journal_title：Journal of immunological methods

authors： Neaga A,Lefor J,Lich KE,Liparoto SF,Xiao YQ

更新日期：2013-04-30 00:00:00

abstract：:The guinea pig has a storied history as a model in the study of infectious disease and immunology. Because of reproducibility of data and availability of various reagents, inbred mice have since supplanted the guinea pig as the animal model-of-choice in these fields. However, several clinically-significant microorgani...

journal_title：Journal of immunological methods

authors： Yu KO,Porcelli SA,Shuman HA

更新日期：2013-03-29 00:00:00

abstract：:Preclinical models of lymph node (LN) metastasis are fundamental to the study and design of new techniques for the diagnosis and treatment of LN metastasis. However, the identification of LNs and lymphatic vessels (LVs) in mice is challenging with conventional imaging modalities, since the LN diameter in normal mice i...

journal_title：Journal of immunological methods

authors： Shao L,Mori S,Yagishita Y,Okuno T,Hatakeyama Y,Sato T,Kodama T

更新日期：2013-03-29 00:00:00

abstract：:Evaluation of human antibody responses to alpha-1 antitrypsin (AAT) in clinical trials and clinical practice has been limited by the lack of a validated assay. Here we describe the development and validation of an ELISA method for quantification of human and nonhuman primate antibody responses to human AAT. A referenc...

journal_title：Journal of immunological methods

authors： Ye GJ,Oshins RA,Rouhani FN,Brantly ML,Chulay JD

更新日期：2013-02-28 00:00:00

abstract：:The aim of this study was to compare the techniques of indirect immunofluorescence assay (IFA) and flow cytometry to clinical and laboratorial evaluation of patients before and after clinical cure and to evaluate the applicability of flow cytometry in post-therapeutic monitoring of patients with American tegumentary l...

journal_title：Journal of immunological methods

authors： de Oliveira AP,de Castro MC,de Almeida AF,Souza Mde A,de Oliveira BC,Reis LC,Goto H,de Brito ME,Celeste BJ,Martins-Filho OA,Pereira VR

更新日期：2013-01-31 00:00:00